u266 b1 human multiple myeloma b lymphocyte cell line  (ATCC)

Journal: Annals of Hematology

Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

doi: 10.1007/s00277-026-06740-8

Figure Lengend Snippet: Identification of constructed TCF4−/+ U266 and MMSA−1−/+ U266 stable cell lines and MMSA-1 mRNA and protein levels in TCF4−/+ U266 cells 1: U266 cells; 2: TCF4− U266-NC; 3. TCF4− U266 cells; 4: TCF4+ U266-NC; 5. TCF4+ U266 cells

Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

Techniques: Construct, Stable Transfection

Journal: Annals of Hematology

Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

doi: 10.1007/s00277-026-06740-8

Figure Lengend Snippet: Dual-Luciferase Report Assay results in different U266 cells

Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

Techniques: Luciferase

Journal: Annals of Hematology

Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

doi: 10.1007/s00277-026-06740-8

Figure Lengend Snippet: MMSA-1 and RAS co-localization and interaction in U266 cells 1: U266 cells; 2: MMSA−1− U266-NC; 3. MMSA−1− U266 cells; 4: MMSA−1+ U266-NC; 5. MMSA−1+ U266 cells

Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

Techniques:

Journal: Annals of Hematology

Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

doi: 10.1007/s00277-026-06740-8

Figure Lengend Snippet: RAS downstream related molecule expressions in different U266 cells 1: U266; 2: MMSA−1− U266-NC; 3. MMSA−1− U266; 4: MMSA−1+ U266-NC; 5. MMSA−1 + U266

Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

Techniques:

Journal: Annals of Hematology

Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

doi: 10.1007/s00277-026-06740-8

Figure Lengend Snippet: MMSA-1’s effect on U266 cells proliferation, migration and apoptosis A showed cloning formation assay result; B showed transwell assay result; C showed apoptosis related proteins expression and D showed mitochondrial apoptosis in different U266 cells. 1: U266; 2: U266 + CCCP-1; 3. MMSA−1− U266-NC; 4. MMSA−1− U266-NC + CCCP-1; 5. MMSA−1− U266; 6: MMSA−1− U266 + CCCP-1; 7. MMSA−1+ U266-NC; 8. MMSA−1+ U266-NC + CCCP-1; 9. MMSA−1 + U266; 10. MMSA−1 + U266 + CCCP-1

Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

Techniques: Migration, Cloning, Tube Formation Assay, Transwell Assay, Expressing

Journal: Annals of Hematology

Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

doi: 10.1007/s00277-026-06740-8

Figure Lengend Snippet: Adhesion molecules and angiogenesis factors levels in different U266 cells A showed qRT-PCR result and B showed Western Blot result of adhesion molecules; C showed angiogenesis factors levels. 1: U266 cells, 2: MMSA−1− U266-NC; 3. MMSA−1− U266; 4. MMSA−1+ U266-NC; 5. MMSA−1+ U266

Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

Techniques: Quantitative RT-PCR, Western Blot

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Identification of novel potent peptide inhibitors targeting the polo-box domain of PLK1: structure-based pharmacophore modelling, virtual screening, molecular docking, molecular dynamics study and biological evaluation

doi: 10.1080/14756366.2025.2563601

Figure Lengend Snippet: Confocal laser scanning microscopy images of U266 cells incubated with FITC-labelled PL-1 for 2 h. Rhodamine-labelled dextran (dextran Rho ) was used as an endocytosis marker, and cell nuclei were stained with Hoechst 33342.

Article Snippet: The human U266 multiple myeloma cells (catalog number: TIB-196) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Confocal Laser Scanning Microscopy, Incubation, Marker, Staining

Journal: PLOS One

Article Title: LC-MS profiling and antioxidant, antifungal, and anticancer potentials of Tunisian Allium sativum L. extracts

doi: 10.1371/journal.pone.0325227

Figure Lengend Snippet: (a) The cytotoxic effects of different solvent extracts Hex (hexane), EtOAc (ethyl acetate), and MeOH (methanol)—were evaluated after 24 h and 48 h of treatment with escalating concentrations (15.56–500 μg/mL). Values are expressed as mean ± SD (n = 3) from three independent assays and presented as percentages relative to the vehicle control. All concentration points were statistically significant compared to the negative control (P < 0.05), except for the first point (15.56 μg/mL) of the hexane and methanol extracts, and the second point of the methanol extract after 48 h of treatment. Statistical comparisons are indicated as follows: a, P < 0.05 ethyl acetate vs. hexane; b, P < 0.05 ethyl acetate vs. methanol; and c, P < 0.05 methanol vs. hexane. (b) Representative phase-contrast inverted microscopy images of U266 cells treated or untreated (vehicle control) with 250 μg/mL of the different extracts (magnification, × 100).

Article Snippet: To study the extracts’ anticancer activities, two cell lines were used: U266 human multiple myeloma cells, generously provided by Professor Brigitte Sola [ ], and MDA-MB-231 , a human breast cancer cell line purchased from ATCC (ATCC HTB-26) [ ].

Techniques: Solvent, Control, Concentration Assay, Negative Control, Inverted Microscopy